文章编号： 1008-0384（2018）09-943-07
Isolation and Expression of Peroxidase POD1 Gene from Cucurbita pepo
HUANG Li-fang 1，2，3 ， LIU Jian-ting 2，3 ， WANG Bin 2，3 ， WEN Qing-fang 2，3 ， ZHU Hai-sheng 2，3* ， LIN Bi-ying1*
（1.College of Horticulture.Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China;
2.Crops Research Institute， Fujian Academy of Agricultural Sciences， Fuzhou， Fujian 350013，China;
3.Vegetable Research Center， Fujian Academy of Agricultural Sciences， Fuzhou， Fujian 350013， China）
Abstract： A functional annotation as peroxidase （POD） gene （i.e.， c47620.graph_c0） was screened from the transcriptomic sequencing of Cucurbita pepo . It was found that thesequence length was 1 245 bp， including a 996bp open reading frame （ORF） encoded a protein of 331 amino acids. The protein had a predicted molecular weight of 35.70 kDa and a hypothetical isoelectric point of 5.40. The similarity of the proteins to the homologous proteins of cucumber， melon and balsam pear were 84%， 84% and 81%， respectively，witha highly conservative nature. It was designated as CpPOD1 gene with a GenBank accession code of MF988285. The RTqPCR revealed the geneto betissue-specific， andits expression in C. pepo highest in the leaves， followed by roots and flowers. Under low temperature and dim light， the CpPOD1 expression was up-regulated over time. It suggested that the gene expression correlatedwith the low temperature/dim light stress as C. pepo regulated the downstream responses. This study could lead to further understanding of the function of CpPOD1 gene inzucchinion cold-and- low-lighttoleranceand the regulation mechanism providing evidence and theoretical basis for improved breeding of C. pepo.
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