摘 要：采用盆栽法研究不同NaCl和Cr3+浓度（分别为0，200，300 mg·kg-1）下葎草幼苗的生长及抗氧化酶活性的变化。结果表明：（1）与对照相比，根、主莖、侧茎的鲜质量和干质量以及SOD活性、Pr含量在NaCl胁迫下显著下降，而POD活性则显著上升，叶鲜质量、主茎长、总叶面积和CAT活性无显著差异；Cr3+胁迫后POD、SOD活性和Pr含量显著上升，CAT活性变化仍无差异，根、主茎、叶的鲜质量和干质量及主茎长、侧茎长均显著降低。（2）与200 mg·kg-1相比，NaCl胁迫后根、主茎、叶的鲜质量仍下降，主茎长、侧茎长、总叶面积、比叶面积、Pr含量和SOD活性的差异不显著，而POD和CAT活性却显著增加； Cr3+胁迫为300 mg·kg-1时，CAT、SOD和POD活性也显著增加，但主茎长却显著减少，其根、茎、叶的鲜质量和Pr含量均无显著差异。另外，根和主茎的干质量、根茎比在二者胁迫后均显著下降。（3）从总体上来看，除NaCl胁迫下主茎长没有显著差异，根、主茎、叶的鲜质量和干质量在二者胁迫后差异显著，其侧茎长、总叶面积、比叶面积和根茎比也具有差异。POD和CAT活性在NaCl和Cr3+胁迫后同时呈上升趋势，SOD活性和Pr含量在Cr3+胁迫后也呈上升趋势，但在NaCl胁迫后则呈下降趋势。因此，NaCl和Cr3+胁迫对葎草幼苗的生长产生了显著影响，其抗氧化酶系统在Cr3+胁迫下具有主动的适应策略，且对NaCl胁迫也表现出较强的耐受性，葎草幼苗对NaCl和Cr3+胁迫具有一定的适应机制和较为积极的响应策略。
关键词：葎草；盐胁迫；铬胁迫；抗氧化酶；生物量
中图分类号：S514 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2017.09.001
Abstract： A pot experiment was conducted to investigate the effect of salinity（NaCl） and chromium （Cr3+） stresses on growth and antioxidant enzyme activities of Humulus scandens seedlings differing in salt and chromium accumulate and tolerance. The results showed that： （1） Compared with the control， the fresh weight and dry weight of the root， main stem， lateral stem and the activity of SOD and the content of Pr under NaCl stress significantly decreased， while the activity of POD increased， the leaf fresh weight， main stem length， total leaf area and CAT activity without significant difference. But the POD， SOD activity and the Pr content under Cr3+ stress increased significantly， while the changes of the root， stem， leaf fresh weight and dry weight and stem length， stem lateral long were significantly lower， the activity of CAT was still no difference. （2）Compared with 200 mg·kg-1， it was still down in the root， stem and leaf fresh weight after NaCl stress， and was no significant changes in the main stem length， lateral stem lengh， total leaf area， Pr content and SOD activity， but the activity of POD and CAT had dramatically increased. When Cr3+ stress was 300 mg·kg-1， there was very markedly risen in CAT， SOD and POD activity， while it was noticeably dropped in the main stem length， and undifferentiated in root， stem and leaf fresh weight and the content of Pr. In addition， dry weight， root and root shoot ratio after stress were also noticeably reduced. 3）On the whole， there were marked effects on root， main stem， leaf fresh weight and dry weight under NaCl and Cr3+ stress except the main stem length， and also obviously different from the lateral stem length， total leaf area， specific leaf area and root to shoot ratio. At the same time， with increasing NaCl and Cr3+ concentration， the CAT and POD presents up-trend， and the POD， SOD activity and the Pr content under Cr3+ stress also showed an upward tendency， but continued to trend downward under NaCl stress. Therefore， Cr3+ and NaCl stress of Humulus scandens seedling growth had a significant impact， the antioxidase system under Cr3+ stress with active adaptation strategies， and under NaCl stress also exhibited a strong tolerance， Humulus scandens seedlings under NaCl and Cr3+ stress has certain indications mechanism and a more positive response strategies.
推荐访问： 幼苗 胁迫 活性 抗氧化 生长
