对照相比，在花后10和20 d时，高温胁迫下SmCCoAOMT的表达量都显著下降，尤其是在花后10 d时，表达量仅为对照的9.23%，说明SmCCoAOMT表达量显著下调可能是导致果皮颜色变浅的原因之一。结果推测SmCCoAOMT在高温胁迫下茄子果皮颜色变浅的过程中起正调控作用。
关键词 茄子;SmCCoAOMT;高温胁迫;果皮着色中图分类号 S641.1 文献标识码 A
SmCCoAOMT Positively Regulates the Peel Coloring in Eggplant under High Temperature Stress
LYU Lingling1， FENG Xuefeng2， LI Ke3， LI Wei3
1. Huaihua University， Huaihua， Hunan 418008， China; 2. Shangqiu University， Shangqiu， Henan 476000， China; 3. South Subtropical Crops Research Institute， Chinese Academy of Tropical Agricultural Sciences / Hainan Provincial Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products， Zhanjiang， Guangdong 524091， China
Abstract Caffeoyl-CoA-O methyltransferase （CCoAOMT） is a key enzyme in the lignin synthetic pathway and also plays an important role in abiotic stresses. According to the eggplant reference genome， SmCCoAOMT was cloned from eggplant. The maximum opening reading frame （ORF） of SmCCoAOMT was 564 bp， encoding 187 aa and its relative molecular weight was 20.95 ku. The bioinformation analysis indicated that SmCCoAOMT showed 90.67% sequence identity with CCoAOMTs of other plants and had all the conservative sequences in CCoAOMT. The expressions in eggplants with different peel colors and at different stages of fruit development were analyzed by qRT-PCR. The results showed that the expression of SmCCoAOMT was significantly higher in purplish red and blackish purple peels than in white and green peels， which was positively correlated with peel color. Comparing to control， on the 10th and 20th days after flowering （10DAF， 20DAF）， the expressions of SmCCoAOMT were significantly down-regulated under high temperature （HT） stess， especially on 10DAF， the expression decreased to 9.23%. Which might lead to the light peel color. The results suggested that SmCCoAOMT positively regulated the peel coloring in eggplant under HT stress.
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