对照组和五味子乙素组，每4 d测量肿瘤体积并绘制肿瘤生长曲线，32 d后取肿瘤称重。Western blot检测MCF-7细胞和肿瘤组织Notch-1及其下游分子Hes-1、Hey-1蛋白水平;免疫组化检测小鼠肿瘤组织Notch-1的表达。结果 与空白组比较，五味子乙素显著抑制MCF-7细胞生长，且抑制作用具有时间和浓度依赖性，同时显著降低MCF-7细胞Notch-1及其下游分子Hes-1、Hey-1蛋白的表达。与对照组比较，五味子乙素组裸鼠肿瘤体积及质量明显受抑制，且肿瘤组织Notch-1及其下游分子Hes-1、Hey-1蛋白表达显著降低。结论 五味子乙素可抑制乳腺癌发展，其机制可能与抑制乳腺癌细胞MCF-7及其裸鼠模型移植瘤的生长、降低Notch-1信号通路相关因子表达有关。
关键词：五味子乙素;细胞增殖;Notch-1;移植瘤;乳腺癌细胞MCF-7;小鼠
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2019）10-0055-05
DOI：10.3969/j.issn.1005-5304.2019.10.013 开放科学（资源服务）标识码（OSID）：
Abstract： Objective To explore the effects of schisandrin B on the growth of MCF-7 and Notch-1 signaling pathway in breast cancer; To explore possible molecular mechanism that inhibit the development of breast cancer. Methods Human breast cancer cell MCF-7 was treated with different concentrations of schisandrin B （0， 20， 40， 80 μmol/L） for 24， 48， 72 h. CCK-8 assay was used to detect cell growth. Nude mice breast cancer xenograft models were established by subcutaneous inoculation of MCF-7 cells in nude mice and were randomly divided into the control group and the schisandrin B treatment group. Tumor volume was measured every 4 days and the tumor growth curve was drawn. After 32 days， the tumor was weighed. The expressions of Notch-1， Hes-1， Hey-1 in MCF-7 cells and tumor tissues were detected by Western blot. Immunohistochemistry was used to detect the expression of Notch-1 in tumor tissues. Results Compared with the blank group， schisandrin B significantly inhibited the growth of MCF-7 cells in a time- and concentration-dependent manner， and significantly decreased the expression of Notch-1 and its downstream molecules Hes-1 and Hey-1 in MCF-7 cells. Compared with the control group， the tumor volume and quality of the schisandrin B treatment group were significantly inhibited， and the expression of Notch-1 and its downstream molecules Hes-1 and Hey-1 significantly decreased. Conclusion Schisandrin B can inhibit the growth of breast cancer， and its mechanism can be related to inhibiting the growth of breast cancer cells MCF-7 and its nude mouse model， and decreasing the expression of Notch-1 signaling pathway.
Keywords： schisandrin B; cell proliferation; Notch-1; xenografted tumor ; breast cancer cell MCF-7; mice
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