中图分类号 R285.5文献标志码 A文章编号 1001-0408（2019）16-2221-05
DOI 10.6039/j.issn.1001-0408.2019.16.12
摘 要 目的：評价安儿宁颗粒的体外抗菌、抗炎和免疫增强活性。方法：采用微量稀释法检测安儿宁颗粒（1.562 5～100 mg/mL）对10种临床常见致病菌的抑制作用并计算其最低抑菌浓度（MIC）;以RAW 264.7细胞（单核巨噬细胞）为对象，采用脂多糖（LPS）诱导建立细胞炎症模型，以MTT法考察安儿宁颗粒（0.312 5～20 mg/mL）对细胞增殖及其释放一氧化氮（NO）的影响;以BALB/c小鼠脾脏淋巴细胞为对象，以MTT法考察安儿宁颗粒（0.312 5～20 mg/mL）对细胞增殖的影响。结果：安儿宁颗粒对表皮葡萄球菌和肺炎链球菌的MIC均为6.25 mg/mL，对变形杆菌、肺炎克雷伯菌、枯草芽孢杆菌和大肠埃希菌（标准菌株）的MIC均为12.5 mg/mL，对铜绿假单胞菌、粪肠球菌、蜡样芽孢杆菌的MIC均为25 mg/mL，对大肠埃希菌（临床分离菌株）、伤寒沙门菌的MIC均为50 mg/mL。与LPS模型组比较，安儿宁颗粒在0.312 5～1.25 mg/mL的剂量下能显著降低LPS诱导的RAW 264.7细胞中NO的释放（P<0.05）;与空白对照组比较，安儿宁颗粒在试验剂量下对RAW 264.7细胞和小鼠脾脏淋巴细胞增殖无干扰或有促增殖趋势，其在0.625、0.312 5 mg/mL剂量下能显著促进淋巴细胞增殖（P<0.05）。结论：安儿宁颗粒在体外对临床常见的革兰氏阳性和革兰氏阴性致病菌均有一定的抑制作用，并具有抗炎和免疫增强作用。
关键词 安儿宁颗粒;抗菌;抗炎;免疫活性
Study on in vitro Antibacterial，Anti-inflammatory and Immunopotentiating Activity of An’erning Granules
LIU Fan1，2，ZHANG Yingying1，HOU Lin1，2（1. College of Pharmacy， Shandong University of TCM， Jinan 250355， China; 2. Qingdao Academy of Chinese Medical Sciences， Shandong University of TCM， Shandong Qingdao 266114， China）
ABSTRACT OBJECTIVE： To evaluate the in vitro antibacterial， anti-inflammatory and immunopotentiating activity of An’erning granules. METHODS： Micro-dilution method was used to determine the minimum inhibitory concentration（MIC）of An’erning granules （1.562 5-100 mg/mL） on 10 kinds of common pathogenic bacteria. Using RAW 264.7 cells （mono-nuclear macrophage） as objects， LPS was used to replicate inflammation model. MTT assay was used to investigate the effects of An’erning granules （0.312 5-20 mg/mL） on proliferation and the release of NO of macrophage. Using spleen lymphocyte of BALB/c mice as objects， MTT assay was used to investigate the effects of An’erning granules （0.312 5-20 mg/mL） on lymphocyte proliferation. RESULTS： MIC of An’erning granules to Epidermis staphylococcus and Streptococcus pneumoniae was 6.25 mg/mL; MIC of An’erning granules to Proteus vulgaris， Klebsiella pneumoniae， Bacillus subtilis and Escherichia coli （standard strain） were 12.5 mg/mL; MIC of An’erning granules to Pseudomonas aeruginosa， Enterococcus faecalis and Bacillus cereus were 25 mg/mL; MIC of An’erning granules to E. coli （clinical isolates） and Salmonella typhia were 50 mg/mL. Compared with LPS model group， An’erning granules could significantly reduce the LPS-induced release of NO in RAW 264.7 cells at the dose of 0.312 5-1.25 mg/mL （P<0.05）. Compared with blank control group， An’erning granule had no interference on the proliferation of RAW 264.7 cells and spleen lymphocytes of mice or had a tendency to promote proliferation. It could significantly promote lymphocyte proliferation at doses of 0.625 and 0.312 5 mg/mL （P<0.05）. CONCLUSIONS： An’erning granules not only have certain in vitro inhibitory effects on clinical common Gram-positive and Gram-negative pathogenic bacteria， but also have anti-inflammatory and immunization enhancement effects.
推荐访问： 体外 抗菌 活性 颗粒 免疫
